Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

HMGA2 (D1A7) Rabbit mAb #8179

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC H M R (Mk) Endogenous 18 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HMGA2 (D1A7) Rabbit mAb recognizes endogenous levels of total HMGA2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HMGA2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HMGA2 (D1A7) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HMGA2 (D1A7) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, HCT 116 (left) or HBP-ALL (right), using HMGA2 (D1A7) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human uterine leiomyoma using HMGA2 (D1A7) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with hTGF-β1 #8915 (10 ng/ml) and hIL-1β #8900 (5 ng/ml, 48 hr; right), using HMGA2 (D1A7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (3). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (4-7). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (8-11).

  1. Cleynen, I. and Van de Ven, W.J. (2008) Int J Oncol 32, 289-305.
  2. Pfannkuche, K. et al. (2009) Stem Cell Rev 5, 224-30.
  3. Monzen, K. et al. (2008) Nat Cell Biol 10, 567-74.
  4. Nishino, J. et al. (2008) Cell 135, 227-39.
  5. Li, O. et al. (2006) Genesis 44, 523-9.
  6. Li, O. et al. (2007) FEBS Lett 581, 3533-7.
  7. Pfannkuche, K. et al. (2009) Stem Cell Rev 5, 224-30.
  8. Fusco, A. and Fedele, M. (2007) Nat Rev Cancer 7, 899-910.
  9. Rawlinson, N.J. et al. (2008) Cancer Genet Cytogenet 181, 119-24.
  10. Wei, J.J. et al. (2010) Am J Surg Pathol 34, 18-26.
  11. Mahajan, A. et al. (2010) Mod Pathol 23, 673-81.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products