Product Pathways - Neuroscience
Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate) #8181
|8181S||100 µl (50 tests)||---||In Stock||---|
|8181||carrier free and custom formulation / quantity||email request|
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Applications Key: IF-F=Immunofluorescence (Frozen)
Specificity / Sensitivity
Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of rat nestin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.
Confocal immunofluorescent analysis of P1 rat cerebellum (left) and adult rat cerebellum (right) using Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate) (red) and Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #8024 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat brain.
Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.