Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Metabolism

FoxA2/HNF3β (D56D6) XP® Rabbit mAb #8186

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Endogenous 50 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FoxA2/HNF3β (D56D6) XP® Rabbit mAb recognizes endogenous levels of total FoxA2/HNF3β protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly138 of human FoxA2/HNF3β protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 cells using FoxA2/HNF3β (D56D6) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Hep G2 (left) and HeLa (right) cells using FoxA2/HNF3β (D56D6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

Forkhead box protein A2 (FoxA2, also known as hepatocyte nuclear factor 3β or HNF3β) is a transcription factor that plays an important role in hepatocyte function (1). FoxA2/HNF3β is required for the activation of hepatic gluconeogenic gene expression during fasting (1). Together with the PGC-1β coactivator, FoxA2/HNF3β stimulates the expression of genes involved in fatty acid β-oxidation and therefore increases fatty acid metabolism (2). FoxA2/HNF3β, along with PGC-1β, also activates the expression of microsomal triacylglycerol transfer protein (MTP) and promotes VLDL secretion (2). In addition to its roles in metabolic syndromes, FoxA2/HNF3β is essential for development of the endoderm and midline structures in mouse embryos (3-5).

  1. Zhang, L. et al. (2005) Cell Metab 2, 141-8.
  2. Wolfrum, C. and Stoffel, M. (2006) Cell Metab 3, 99-110.
  3. Levinson-Dushnik, M. and Benvenisty, N. (1997) Mol Cell Biol 17, 3817-22.
  4. Weinstein, D.C. et al. (1994) Cell 78, 575-88.
  5. Ang, S.L. and Rossant, J. (1994) Cell 78, 561-74.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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