Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

DDX3 (D19B4) Rabbit mAb #8192

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 75 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DDX3 (D19B4) Rabbit mAb recognizes endogenous levels of total DDX3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DDX3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DDX3 (D19B4) Rabbit mAb.

Background

The DEAD box family of RNA helicases is characterized in part by a common D-E-A-D amino acid motif. The family is composed of a growing number of proteins found in a wide range of organisms from bacteria to mammals. DEAD helicases have distinct biological functions in RNA metabolism and ribonucleoprotein (RNP) processing (reviewed in 1,2).DDX3 is a DEAD box family RNA helicase with diverse cellular functions. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1 (3). DDX3 is required for hepatitis C virus (HCV) RNA replication (4) and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC) (5).Recent evidence suggests that DDX3 functions as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC (5,6), and aberrant subcellular localization occurs in many squamous cell carcinomas (6). Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression (5).DDX3 is phosphorylated at Thr204 and Thr323 by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation is thought to cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis (7).

  1. Rocak, S. and Linder, P. (2004) Nat Rev Mol Cell Biol 5, 232-41.
  2. Linder, P. (2006) Nucleic Acids Res 34, 4168-80.
  3. Yedavalli, V.S. et al. (2004) Cell 119, 381-92.
  4. Ariumi, Y. et al. (2007) J Virol 81, 13922-6.
  5. Chang, P.C. et al. (2006) Oncogene 25, 1991-2003.
  6. Chao, C.H. et al. (2006) Cancer Res 66, 6579-88.
  7. Sekiguchi, T. et al. (2007) Biochem Biophys Res Commun 356, 668-73.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products