Cell Signaling Technology

Product Pathways - Adhesion

ZO-1 (D7D12) Rabbit mAb #8193

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 220 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ZO-1 (D7D12) Rabbit mAb recognizes endogenous levels of total ZO-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZO-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ZO-1 (D7D12) Rabbit mAb.

IP

IP

Immunoprecipitation and western blot analysis of extracts from Hep G2 cells using ZO-1 (D7D12) Rabbit mAb. Lane 1 is 10% input.

Background

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).

  1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
  2. Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11.
  3. Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47.
  4. Umeda, K. et al. (2006) Cell 126, 741-54.
  5. Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66.
  6. Traweger, A. et al. (2003) J Biol Chem 278, 2692-700.
  7. Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36.
  8. Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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