Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Mouse Lipocalin-2 (mLCN2) #8199

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Source

Recombinant mouse LCN2 (mLCN2) Gln21-Asn200 (Accession #P11672) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant mLCN2 contains no "tags" and the nonglycosylated protein has a calculated MW of 20,874. DTT-reduced and non-reduced proteins migrate as 24 kDa polypeptides.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mLCN2 All lots are greater than 98% pure.

Bioactivity

The bioactivity of mLCN2 was determined in a BAF3 cell viability assay. The ED50 of each lot is between 0.5-4 µg/ml.

Coomassie Gel

Coomassie Gel

The purity of recombinant mLCN2 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mLCN2 and staining overnight with Coomassie Blue.

Bioactivity

Bioactivity

The viability of BAF3 cells treated with increasing amounts of mLCN2 was assessed. After 48 hr treatment with mLCN2, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.

Endotoxin

Less than 0.01 ng endotoxin/1 μg mLCN2.

Formulation

With carrier: A 0.22 μm filtered solution of 0.33 mg/ml mLCN2 in 20 mM Tris, pH 8.0 containing 150 mM NaCl and 20 μg BSA per 1 μg mLCN2. Carrier free: A 0.22 μm filtered solution of 0.33 mg/ml mLCN2 in 20 mM Tris, pH 8.0 containing 150 mM NaCl.

Background

Lipocalin-2, a member of the lipocalin family of proteins, was originally identified as a gelatinase-associated component of neutrophil secretory granules (1). Lipocalin-2 is involved in innate immunity, iron homeostasis, and apoptosis. Lipocalin-2 limits bacterial growth by binding to bacterial siderophores and sequestering iron (2-4). In mammalian cells, iron-loaded lipocalin-2 binds to its receptor, 24p3R, and is internalized, thereby releasing iron and increasing the intracellular iron concentration (5). Conversely, iron-free lipocalin-2 promotes apoptosis (5). Lipocalin-2 is also expressed in adipose tissue and promotes insulin resistance in cultured mouse adipocytes (6).

  1. Kjeldsen, L. et al. (1994) Blood 83, 799-807.
  2. Flo, T.H. et al. (2004) Nature 432, 917-21.
  3. Goetz, D.H. et al. (2002) Mol Cell 10, 1033-43.
  4. Bachman, M.A. et al. (2009) PLoS Pathog 5, e1000622.
  5. Devireddy, L.R. et al. (2005) Cell 123, 1293-305.
  6. Yan, Q.W. et al. (2007) Diabetes 56, 2533-40.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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