Product Pathways - Apoptosis
PhosphoPlus® Caspase-3 (Cleaved, Asp175) Antibody Duet #8202
|Duet Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664||100 µl||W IP IHC-P IHC-F IF-IC F||H M R Mk (Dg)||17, 19||Rabbit IgG|
|Caspase-3 (8G10) Rabbit mAb #9665||100 µl||W IP||H M R Mk||17, 19, 35||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dg=Dog
Species in parentheses are predicted to react based on 100% sequence homology.
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
- Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761-30764.
- Nicholson, D. W. et al. (1995) Nature 376, 37-43.
For Research Use Only. Not For Use In Diagnostic Procedures.