Product Pathways - Jak/Stat Pathway
PhosphoPlus® Stat3 (Tyr705) Antibody Duet #8204
|Duet Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145||100 µl||W IP IHC-P IHC-F IF-IC F ChIP||H M R Mk (Hm) (B) (Pg) (Hr)||79, 86||Rabbit IgG|
|Stat3 (79D7) Rabbit mAb #4904||100 µl||W IP ChIP||H M R Mk||79, 86||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey B=Bovine Pg=Pig Hr=Horse
Species in parentheses are predicted to react based on 100% sequence homology.
* Product-specific protocol.
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
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For Research Use Only. Not For Use In Diagnostic Procedures.