Product Pathways - Translational Control
PhosphoPlus® S6 Ribosomal Protein (Ser235/Ser236) Antibody Duet #8207
| Duet Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 | 100 µl | W IHC-P IHC-F IF-IC F | H M R Mk Sc (C) | 32 | Rabbit IgG |
| S6 Ribosomal Protein (5G10) Rabbit mAb #2217 | 100 µl | W IHC-P IF-F IF-IC | H M R Mk | 32 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
Sc=S. cerevisiae
Species in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2217:
- IHC / Paraffin, Immunofluorescence, Western Blotting
- 4858:
- Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Western Blotting
Description
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Background
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
- Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
- Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
- Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
- Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
- Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
