Product Pathways - MAPK Signaling
PhosphoPlus® MEK1/2 (Ser217/Ser221) Antibody Duet #8211
|Duet Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|MEK1/2 (D1A5) Rabbit mAb #8727||100 µl||W IF-IC F||H M R Mk (Hm) Dm (X) (Z) (B) (Dg) (Pg) (Ce)||46||Rabbit IgG|
|Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154||100 µl||W IP||H M R Mk Dm (C)||45||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Dm=D. melanogaster X=Xenopus Z=Zebrafish B=Bovine Dg=Dog Pg=Pig Ce=C. elegans
Species in parentheses are predicted to react based on 100% sequence homology.
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Crews, C.M. et al. (1992) Science 258, 478-480.
- Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
- Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
- Cowley, S. et al. (1994) Cell 77, 841-852.
For Research Use Only. Not For Use In Diagnostic Procedures.