Product Pathways - NF-kB Signaling
PhosphoPlus® NF-κB p65/RelA (Ser536) Antibody Duet #8214
| Duet Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 | 100 µl | W IP IF-IC F | H M R Hm Mk Pg (Dg) | 65 | Rabbit IgG |
| NF-κB p65 (D14E12) XP® Rabbit mAb #8242 | 100 µl | W IP IHC-P IF-IC F ChIP | H M R Hm Mk Dg | 65 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
ChIP=Chromatin IP
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
Dg=Dog
Pg=Pig
Species in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3033:
- Flow, Immunofluorescence, Immunoprecipitation, Western Blotting
- 8242:
- ChIP Agarose, ChIP Magnetic, Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
Description
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Background
Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).
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For Research Use Only. Not For Use In Diagnostic Procedures.
