Cell Signaling Technology

Product Pathways - Development

PhosphoPlus® Notch1 (Cleaved, Val1744) Antibody Duet #8216

Duet Includes Quantity Applications Reactivity MW (kDa) Isotype
Notch1 (D1E11) XP® Rabbit mAb #3608 100 µl W IHC-P ChIP H M R 120, 300 Rabbit IgG
Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147 100 µl W IP ChIP H M R 110 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  ChIP=Chromatin IP
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species in parentheses are predicted to react based on 100% sequence homology.

Protocols

Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).

  1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
  2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
  3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
  4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.

For Research Use Only. Not For Use In Diagnostic Procedures.

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