Product Pathways - Jak/Stat Pathway
PhosphoPlus® Stat1 (Tyr701) Antibody Duet #8217
|8217S||1 Kit (10 western blots)||---||In Stock||---|
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|Duet Includes||Quantity||Applications||Reactivity||Homology†||Mol. Wt.||Isotype|
|Stat1 (42H3) Rabbit mAb #9175||100 µl||W, IHC-P||Human, Monkey||84, 91 kDa||Rabbit IgG|
|Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649||100 µl||W, IP, IF-IC, F, ChIP||Human, Mouse, Rat||Monkey||84, 91 kDa||Rabbit IgG|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP
* Product-specific protocol.
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
For Research Use Only. Not For Use In Diagnostic Procedures.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.