Product Pathways - NF-kB Signaling
PhosphoPlus® IκBα (Ser32/Ser36) Antibody Duet #8219
|Duet Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246||100 µl||W||H M R Mk (B) (Dg) (Pg) (GP)||40||Mouse IgG1|
|IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814||100 µl||W IP IHC-P IF-IC F||H M R Mk B Pg GP||39||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine Dg=Dog Pg=Pig GP=Guinea Pig
Species in parentheses are predicted to react based on 100% sequence homology.
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
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For Research Use Only. Not For Use In Diagnostic Procedures.