Product Pathways - NF-kB Signaling
PhosphoPlus® IκBα (Ser32/Ser36) Antibody Duet #8219
| Duet Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246 | 100 µl | W IP IHC-P | H M R Mk Dg (B) (Pg) (GP) | 40 | Mouse IgG1 |
| IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 | 100 µl | W IP IHC-P IF-IC F | H M R Mk B Pg GP | 39 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Dg=Dog
Pg=Pig
GP=Guinea Pig
Species in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4814:
- Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
- 9246:
- IHC / Paraffin, Immunoprecipitation, Western Blotting
Description
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
- Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
