Cell Signaling Technology

Product Pathways - MAPK Signaling

PhosphoPlus® ATF-2 (Thr71) Antibody Duet #8220

Duet Includes Quantity Applications Reactivity MW (kDa) Isotype
Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112 100 µl W F H M R Mk 70 Rabbit IgG
ATF-2 (20F1) Rabbit mAb #9226 100 µl W IP IHC-P H M R Mk 65 to 75 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species in parentheses are predicted to react based on 100% sequence homology.

Protocols

Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

  1. Abdel-Hafiz, H.A. et al. (1992) Mol. Endocrinol. 6, 2079-2089.
  2. Gupta, S. et al. (1995) Science 267, 389-393.
  3. van Dam, H. et al. (1995) EMBO J. 14, 1798-1811.
  4. Livingstone, C. et al. (1995) EMBO J. 14, 1785-1797.

For Research Use Only. Not For Use In Diagnostic Procedures.

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