Product Pathways - Jak/Stat Pathway
PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet #8224
| Duet Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776 | 100 µl | W | H M R (Mk) (B) | 125 | Rabbit IgG |
| Jak2 (D2E12) XP® Rabbit mAb #3230 | 100 µl | W IP IHC-P IF-IC F | H M R (Hm) (Mk) (C) (X) (B) (Dg) (Pg) (Hr) | 125 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
C=Chicken
X=Xenopus
B=Bovine
Dg=Dog
Pg=Pig
Hr=Horse
Species in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3230:
- Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
- 3776:
- Western Blotting
Description
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-5.
- VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
- Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
- Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
