Product Pathways - Development
CACYBP (D43G11) Rabbit mAb #8225
|8225S||100 µl (10 western blots)||---||In Stock||---|
|8225||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||26||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
Specificity / Sensitivity
CACYBP (D43G11) Rabbit mAb recognizes endogenous levels of total CACYBP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg162 of human CACYBP protein.
Western blot analysis of extracts from various cell lines using CACYBP (D43G11) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CACYBP (D43G11) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymphoma using CACYBP (D43G11) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded colon carcinoma using CACYBP (D43G11) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Calcyclin-binding protein or Siah-1-interacting protein (CACYBP/SIP) is a component of the ubiquitin E3 ligase complex that also contains Siah1, Skp1, and Ebi (1). CACYBP regulates β-catenin turnover and plays an important role in thymocyte development (2). CACYBP also binds to tubulin and may be involved in cytoskeletal regulation (3,4). It is highly expressed in neurons, and its cellular localization may be regulated by Ca2+ (5,6). Retinoic acid treatment of the neuroblastoma cell line SH-SY5Y induces translocation of CACYBP to the nucleus and seems to be correlated with phosphorylation of CACYBP on serine residues (7). Recent studies also suggest that CACYBP may possess phosphatase activity (8), and that it can bind and dephosphorylate Erk1/2 (8,9).
- Matsuzawa, S.I. and Reed, J.C. (2001) Mol Cell 7, 915-26.
- Fukushima, T. et al. (2006) Immunity 24, 29-39.
- Schneider, G. et al. (2007) Biochim Biophys Acta 1773, 1628-36.
- Filipek, A. et al. (2008) J Neural Transm 115, 1257-64.
- Jastrzebska, B. et al. (2000) J Histochem Cytochem 48, 1195-202.
- Filipek, A. et al. (2002) J Biol Chem 277, 21103-9.
- Wu, J. et al. (2003) J Biochem Mol Biol 36, 354-8.
- Kilanczyk, E. et al. (2011) Biochem Biophys Res Commun 404, 179-83.
- Kilanczyk, E. et al. (2009) Biochem Biophys Res Commun 380, 54-9.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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