Product Pathways - Protein Stability
HIP2 (D27C4) Rabbit mAb #8226
|W IP||H M R Mk (B)||Endogenous||25||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
HIP2 (D27C4) Rabbit mAb recognizes endogenous levels of total HIP2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu161 of human HIP2 protein.
Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).
Huntingtin-interacting protein-2 (HIP2), also known as E2-25K, is a member of the E2 protein family that is highly expressed in the brain and catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin (2). E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-β, huntingtin, and MHC-heavy chain proteins (3-6). Recent studies have also implicated HIP2 in the control of apoptosis and cell-cycle progression through its ability to regulate the stability of Smac/Diablo and cyclin B1 (7,8).
- Hershko, A. (1988) J. Biol. Chem. 263, 15237-15240.
- Chen, Z. and Pickart, C.M. (1990) J Biol Chem 265, 21835-42.
- Song, S. et al. (2003) Mol Cell 12, 553-63.
- de Pril, R. et al. (2007) Mol Cell Neurosci 34, 10-9.
- Flierman, D. et al. (2006) Proc Natl Acad Sci USA 103, 11589-94.
- Kalchman, M.A. et al. (1996) J Biol Chem 271, 19385-94.
- Bae, Y. et al. (2010) Biochem Biophys Res Commun 397, 718-23.
- Bae, Y. et al. (2010) FEBS Lett 584, 4505-10.
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For Research Use Only. Not For Use In Diagnostic Procedures.