Product Pathways - Stem Cell and Lineage Markers
DDX4 Antibody #8227
|8227S||100 µl (10 western blots)||---||In Stock||---|
|8227||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||80||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen)
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
DDX4 Antibody detects endogenous levels of total DDX4 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly265 of human DDX4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer, suggesting a role in microRNA-mediated RNA silencing (1).
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