Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

AML1 Antibody (Mouse Preferred) #8229

Applications Reactivity Sensitivity MW (kDa) Source
W H M (R) (Mk) Endogenous 55 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

AML1 Antibody (Mouse Preferred) detects endogenous levels of total AML1 protein. This antibody will recognize human AML1, however, we recommend AML1 (D33G6) XP® Rabbit mAb #4336 for detection of human AML1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val211 of human AML1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using AML1 Antibody (Mouse Preferred).

Background

AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).

  1. Wang, S. et al. (1993) Mol Cell Biol 13, 3324-3339.
  2. Ogawa, E. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 6859-6863.
  3. Okuda, T. et al. (1996) Cell 84, 321-30.
  4. Wang, Q. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 3444-3449.
  5. North, T.E. et al. (2004) Stem Cells 22, 158-168.
  6. Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-387.
  7. Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
  8. Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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