Product Pathways - Growth Factors/Cytokines
Human Lymphotoxin-α/TNF-β/TNFSF1 (hLT-α) #8230
PhosphoSitePlus® protein, site, and accession data: LTA
Request pricing for multi-milligram amounts.
Source
Recombinant human LT-α (hLT-α) Pro36-Leu205 (Accession #NP_000586) was expressed in human 293 cells at Cell Signaling Technology.
Molecular Characterization
Recombinant hLT-α contains no "tags" and the nonglycosylated protein has a calculated MW of 18,548. DTT-reduced and non-reduced protein migrate as 24-31 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal PGVGL of recombinant hLT-α was verified by amino acid sequencing.
Purity
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hLT-α. All lots are greater than 98% pure.
Bioactivity
The bioactivity of recombinant hLT-α was determined in an L-929 cell viability assay. The ED50 of each lot is between 15-150 pg/ml.
Coomassie Gel
The purity of recombinant hLT-α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hLT-α and staining overnight with Coomassie Blue.
Bioactivity
The viability of L-929 cells treated with increasing amounts of hLT-α in the presence of 2 ng/ml actinomycin D was determined. After 24 hour treatment with hLT-α, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.
Endotoxin
Less than 0.01 ng endotoxin/1 μg hLT-α.
Formulation
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hLT-α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Background
Lymphotoxin-α (LT-α), also known as TNF–β, is a member of the TNF superfamily of proteins (1). NK cells, T cells, B cells, and lymphoid tissue-inducer cells express LT-α (1). LT-α can be secreted as a soluble homotrimer or form membrane bound heterotrimers with lymphotoxin-β (LT α1β2 or LT α2β1) which can be cleaved from the cell surface by matrix metalloproteases (1,2). Soluble LT-α binds to and signals through TNFR1/TNFR2, activating the canonical NF-κB pathway (1). In contrast, LT α1β2 heterodimers bind to the LTβR receptor and activate the noncanonical NF-κB pathway (1). As a result, LT-α and TNF-α have overlapping functions. Soluble LT-α and LT α1β2 play key roles in lymphangiogenesis (3). The LT α1β2/LTβR axis is essential for the development of lymphoid tissue (1,3).
- Wolf, M.J. et al. (2010) Oncogene 29, 5006-18.
- Young, J. et al. (2010) Cytokine 51, 78-86.
- Mounzer, R.H. et al. (2010) Blood 116, 2173-82.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- 9872 Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile)
For Research Use Only. Not For Use In Diagnostic Procedures.
