Cell Signaling Technology

Product Pathways - Neuroscience

Homer1 Antibody #8231

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 46 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Homer1 Antibody detects endogenous levels of total Homer1 protein. This antibody may also detect isoform Homer1c.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu130 of human Homer1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain tissue using Homer1 Antibody.

Background

Homer1, Homer2 and Homer3 are scaffolding proteins, composed of an EVH protein–binding domain, a coiled-coil domain and a leucine zipper domain. The EVH domain is a protein-protein binding module that binds to the proline-rich motifs PPXXF, PPXF, and LPSSP of G protein–coupled receptors (GPCRs), inositol 1,4,5-triphosphate (IP3) receptors (IP3Rs), ryanodine receptors, and TRP channels (1-2). The coiled-coil and the leucine zipper domains cause multimerization of Homers and assemble signaling proteins complexes. The Homer1 gene encodes a short isoform (Homer1a, aa 1-186) and two long isoforms (Homer1b, aa 1-354; Homer1c, aa 1-366). Homer1a lacks the coiled-coil domain and leucine zipper, antagonizing multimerization of Homers and thus disassembling signaling proteins complexes (3).

  1. Fagni, L. et al. (2002) Sci STKE 2002, re8.
  2. Yuan, J.P. et al. (2003) Cell 114, 777-89.
  3. Xiao, B. et al. (2000) Curr Opin Neurobiol 10, 370-4.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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