Product Pathways - Metabolism

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Glucagon (D16G10) XP^® Rabbit mAb #8233

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
IHC-P IHC-F IF-F	H M R	Endogenous	4	Rabbit IgG

Applications Key:  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

8233:

IHC / Frozen, IHC / Paraffin, Immunofluorescence

Specificity / Sensitivity

Glucagon (D16G10) XP^® Rabbit mAb recognizes endogenous levels of total glucagon protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala137 of human glucagon protein.

Background

Glucose homeostasis is regulated by a variety of hormones including glucagon. Glucagon is synthesized as the precursor molecule proglucagon and is proteolytically processed to yield the mature peptide in α cells of the pancreatic islets. Glucagon causes the release of glucose from glycogen and stimulates gluconeogenesis in the liver (1,2).

1. Hers, H.G. and Hue, L. (1983) Annu. Rev. Biochem. 52, 617-653.
2. Granner, D. and Pilkis, S. (1990) J. Biol. Chem. 265, 10173-10176.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 2760 Glucagon Antibody
• 3014 Insulin (C27C9) Rabbit mAb
• 4590 Insulin Antibody
• 4593 C-Peptide Antibody
• 8112 SignalStain^® Antibody Diluent
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 5425 Normal Goat Serum
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

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For Research Use Only. Not For Use In Diagnostic Procedures.