Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

ROCK2 Antibody #8236

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R (Mk) Endogenous 160 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ROCK2 Antibody recognizes endogenous levels of total ROCK2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ROCK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ROCK2 Antibody.

IP

IP

Immunoprecipitation of ROCK2 from HT-29 cells using ROCK2 Antibody. Western blot was performed using the same antibody. The (-) lane is 10% input.

Background

ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (4-6).

  1. Nakagawa, O. et al. (1996) FEBS Lett. 392, 189-193.
  2. Lee, J.H. et al. (2004) J. Cell. Biol. 167, 327-337.
  3. Sebbagh, M. et al. (2005) J. Exp. Med. 201, 465-471.
  4. Amano, M. et al. (1996) J. Biol. Chem. 271, 20246-20249.
  5. Kureishi, Y. et al. (1997) J. Biol. Chem. 272, 12257-12260.
  6. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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