Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Neuroscience

p75NTR (D4B3) XP® Rabbit mAb #8238

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Endogenous 75 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

p75NTR (D4B3) XP® Rabbit mAb recognizes endogenous levels of total p75NTR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg198 of human p75NTR protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from PC-3 cells, untreated (-) or ibuprofen-treated (2 mM, 24 hr) (+), and SW480 cells (-) using p75NTR (D4B3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-N-MC cells using p75NTR (D4B3) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

The p75 neurotrophin receptor (p75NTR), a member of the TNF receptor superfamily, is distinguished by multiple cysteine-rich ligand-binding domains, a single transmembrane sequence and a noncatalytic cytoplasmic domain (1). p75NTR displays paradoxical functions when acting alone or with other receptor proteins. Working in concert with Trk receptors, p75NTR recognizes neurotrophins and transmits trophic signals into the cell. Both p75NTR and TrkA are required to activate PI3K-Akt signaling, while TrkA can individually activate the MAP kinase pathway. In contrast, p75NTR, possibly through JNK, ensures appropriate apoptosis of injured neurons and improperly targeted neonatal neurons (2).The p75NTR protein undergoes sequential cleavage similar to APP and Notch. First, α-secretase removes the p75NTR ectodomain, eliminating ligand-mediated signaling. At this point, the membrane-tethered cleavage product can still fine-tune Trk-mediated trophic actions. γ-secretase cleaves within the transmembrane domain to liberate the cytoplasmic tail from its membrane anchor and allow the p75NTR intracellular domain to translocate to the nucleus (3,4).

  1. Chao, M.V. (2003) Nat. Rev. Neurosci. 4, 299-309.
  2. Nykjaer, A. et al. (2005) Curr. Opin. Neurobiol. 15, 49-57.
  3. Kanning, K.C. et al. (2003) J. Neurosci. 23, 5425-5436.
  4. Jung, K.M. et al. (2003) J. Biol. Chem. 278, 42161-42169.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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