Cell Signaling Technology

Product Pathways - Protein Folding

FKBP5 Antibody #8245

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M Mk Endogenous 51 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FKBP5 Antibody recognizes endogenous levels of total FKBP5 protein. This antibody does not cross-react with FKBP4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FKBP5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and SH-SY5Y cells using FKBP5 Antibody.

Background

FK506 binding protein 51 (FKBP51, also called FKBP5) belongs to the FKBP family of immunophilins (1). FKBP family proteins contain FK domains and TPR (tetratricopeptide repeat) domains. The FK domains are responsible for PPIase (peptidylprolyl isomerase) acitivity and allow binding to FK506 and rapamycin (2,3). The C terminal TPR domains are involved in protein-protein interactions. The TPR domain of FKBP5 mediates binding to HSP90 complexes (4), as well as glucocorticoid, androgen, and progesterone receptors, which account for its regulatory role in steroid hormone receptor function (5). FKBP5 also binds to IKKα and is involved in NF-κB signaling (6,7). In addition, FKBP5 was identified as a negative regulator of Akt, through promotion of Akt - PHLPP interaction and enhanced dephosphorylation of Akt (8).

  1. Avilés, A. et al. (1995) Clin Oncol (R Coll Radiol) 7, 97-101.
  2. Fruman, D.A. et al. (1994) FASEB J 8, 391-400.
  3. Fix, M.L. et al. (2008) Blood Press Monit 13, 199-203.
  4. Scheufler, C. et al. (2000) Cell 101, 199-210.
  5. Schülke, J.P. et al. (2010) PLoS One 5, e11717.
  6. Bouwmeester, T. et al. (2004) Nat Cell Biol 6, 97-105.
  7. Avellino, R. et al. (2005) Blood 106, 1400-6.
  8. Pei, H. et al. (2009) Cancer Cell 16, 259-66.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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