Cell Signaling Technology

Product Pathways - Phosphatases

PTPN18 (D7B9) Rabbit mAb #8311

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 48 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PTPN18 (D7B9) Rabbit mAb recognizes endogenous levels of total PTPN18 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala401 of human PTPN18 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PTPN18 (D7B9) Rabbit mAb.

Background

PTPN18 is a member of the PEST family of nonreceptor protein tyrosine phosphatases, a group that also includes PTP-PEST and PEP (1-3). Members of this protein family contain an N-terminal catalytic domain and a noncatalytic C-terminal domain with a PEST motif that mediates protein-protein interactions (4). PTPN18 was first identified in hematopoietic stem cells (HSCs), where it was suggested to play a role in HSC differentiation (1-3). Subsequently, PTPN18 was shown to bind to c-Src kinase, forming a protein complex that functions to inhibit Src-family kinase signaling (4,5). More recently, PTPN18 expression has been shown to correlate with HER2 receptor expression in breast cancer cell lines (6,7), where it has been suggested to repress HER2 signaling (7).

  1. Cheng, J. et al. (1996) Blood 88, 1156-67.
  2. Huang, K. et al. (1996) Oncogene 13, 1567-73.
  3. Dosil, M. et al. (1996) Blood 88, 4510-25.
  4. Wang, B. et al. (2001) Mol Cell Biol 21, 1077-88.
  5. Rubbi, L. et al. (2011) Sci Signal 4, ra18.
  6. Lucci, M.A. et al. (2010) Cell Oncol 32, 361-72.
  7. Gensler, M. et al. (2004) J Biol Chem 279, 12110-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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