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Fatty Acid and Lipid Metabolism Antibody Sampler Kit #8335

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
AceCS1 (D19C6) Rabbit mAb #3658	40 µl	W IP	H M R Mk	78	Rabbit IgG
Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676	40 µl	W IHC-P IF-IC F	H M R Hm	280 kDa	Rabbit IgG
Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661	40 µl	W IP IHC-P	H M R Mk (C) (B)	280	Rabbit
ATP-Citrate Lyase Antibody #4332	40 µl	W	H M R Mk	125	Rabbit
Phospho-ATP-Citrate Lyase (Ser455) Antibody #4331	40 µl	W IP	H M (R)	125	Rabbit
Fatty Acid Synthase (C20G5) Rabbit mAb #3180	40 µl	W IP IHC-P IHC-F IF-IC	H M R (B)	273	Rabbit IgG
Lipin 1 Antibody #5195	40 µl	W IP IF-IC	H M	130	Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat
ACSL1 (D2H5) Rabbit mAb #9189	40 µl	W IP	H M R Mk	78	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

AceCS1 (D19C6) Rabbit mAb recognizes endogenous levels of total cytoplasmic acetyl-CoA synthetase. Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody recognizes endogenous levels of ACC only when phosphorylated at Ser79; this antibody recognizes both ACCα and ACCß. Acetyl-CoA Carboxylase (C83B10) Rabbit mAb recognizes endogenous levels of all isoforms of acetyl-CoA carboxylase protein. Phospho-ATP-Citrate Lyase (Ser455) Antibody recognizes endogenous levels of ATP-citrate lyase only when phosphorylated at Ser455. ACSL1 (D2H5) Rabbit mAb, ATP-Citrate Lyase Antibody, Fatty Acid Synthase (C20G5) Rabbit mAb, and Lipin 1 Antibody recognize endogenous levels of their respective target proteins.

Description

The Fatty Acid and Lipid Metabolism Antibody Sampler Kit provides an economical means to evaluate key proteins involved in fatty acid and lipid metabolism. This kit includes enough primary antibody to perform four western miniblot experiments with each primary antibody.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human cytoplasmic acetyl-CoA synthetase, residues surrounding Ser523 of human acetyl-CoA carboxylase α1, residues surrounding Gly46 of human fatty acid synthase or residues surrounding Ala257 of human ACSL1 protein. Modification state-specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of rat ACC protein or residues surrounding Ser455 of human ATP-citrate lyase protein. Polyclonal antibodies are produced by immunizing animals corresponding to residues of human ATP-citrate lyase protein, or corresponding to residues surrounding Gln883 of human Lipin 1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

The processes of fatty acid and lipid metabolism are vital for cellular nutrient and energy maintenance. Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA. Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis (1,2). Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (4). ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol, and acetylcholine, as well as for glucogenesis (5). Phosphorylation of ACL at Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (6). Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA (7). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (8-10). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in the liver and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (11).

1. Ikeda, Y. et al. (2001) J Biol Chem 276, 34259-69.
2. Luong, A. et al. (2000) J Biol Chem 275, 26458-66.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Mashek, D.G. et al. (2004) J Lipid Res 45, 1958-61.
5. Towle, H.C. et al. (1997) Annu Rev Nutr 17, 405-33.
6. Potapova, I.A. et al. (2000) Biochemistry 39, 1169-79.
7. Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33.
8. Finck, B.N. et al. (2006) Cell Metab 4, 199-210.
9. Phan, J. and Reue, K. (2005) Cell Metab 1, 73-83.
10. Verheijen, M.H. et al. (2003) Genes Dev 17, 2450-64.
11. Reue, K. and Zhang, P. (2008) FEBS Lett 582, 90-6.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

3180:

IHC / Frozen, IHC / Paraffin, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

3658:

Immunoprecipitation, Western Blotting

3661:

IHC / Paraffin, Immunoprecipitation, Western Blotting

3676:

Flow, IHC / Paraffin, Immunofluorescence, Western Blotting

4331:

Immunoprecipitation, Western Blotting

4332:

Western Blotting

5195:

Immunofluorescence, Immunoprecipitation, Western Blotting

9189:

Immunoprecipitation, Western Blotting

Companion Products

• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9998 BSA
• 9999 Nonfat Dry Milk
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
• 7722 Blue Loading Buffer Pack
• 7723 Red Loading Buffer Pack

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For Research Use Only. Not For Use In Diagnostic Procedures.