Product Pathways - Apoptosis
Death Receptor Antibody Sampler Kit #8356
|8356S||1 Kit (9 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Fas (C18C12) Rabbit mAb #4233||40 µl||W, IHC-P||H||40-50||Rabbit IgG|
|TNF-R1 (C25C1) Rabbit mAb #3736||40 µl||W, IP||H||55||Rabbit IgG|
|TNF-R2 Antibody #3727||40 µl||W, IP||H, M, R||Mk||65||Rabbit|
|DR3 Antibody #3254||40 µl||W||H||48||Rabbit|
|DR5 (D4E9) XP® Rabbit mAb #8074||40 µl||W, IP, IF-IC||H||40, 48||Rabbit IgG|
|FADD Antibody (Human Specific) #2782||40 µl||W||H||28||Rabbit|
|TRADD (7G8) Rabbit mAb #3684||40 µl||W, IP||H||32||Rabbit IgG|
|DcR3 Antibody #4758||40 µl||W||H, R||32||Rabbit|
|RIP (D94C12) XP® Rabbit mAb #3493||40 µl||W, IP, IF-IC, F||H, M, R, Hm, Mk||78||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey
Western blot analysis of extracts from Jurkat and HeLa cells, treated with 4 mM hydroxyurea or 1 µg/ml nocodazole (each for 20 hours), using FADD Antibody (Human Specific) #2782.
Western blot analysis of extracts from various cell lines using RIP (D94C12) XP® Rabbit mAb #3493.
Western blot analysis of extracts from various cell lines using TRADD (7G8) Rabbit mAb #3684.
Western blot analysis of extracts from various cell lines using TNF-R2 Antibody #3727.
Western blot analysis of extracts from various cell lines using TNF-R1 (C25C1) Rabbit mAb #3736.
Western blot analysis of extracts from COS7 cells, mock transfected or transfected with human Fas, and from ACHN and HT-1080 cell lines, using Fas (C18C12) Rabbit mAb #4233.
Western blot analysis of extracts from various cell lines using DcR3 Antibody #4758.
Western blot analysis of extracts from various cell lines using DR5 (D4E9) XP® Rabbit mAb #8074.
The Death Receptor Antibody Sampler Kit provides an economical means to investigate the machinery of death receptor-mediated apoptosis. The kit includes enough of each primary antibody to perform four western mini-blot experiments per primary.
Specificity / Sensitivity
Each antibody in the Death Receptor Antibody Sampler Kit recognizes endogenous levels of the respective target protein. The TNF-R1, DR3, DR5, and RIP antibodies do not cross-react with other related family members. TNF-R1 C25C1 Rabbit mAb may recognize a 30 kDa splice isoform of TNF-R1 in some cell lines.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Lys259 of human Fas, Ser331 of human TNF-R1, Arg260 of human DR5 protein, Gly227 of human TRADD, and Leu190 of human RIP, respectively. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Asp335 human TNF-R2, Pro310 of human DR3, Ser194 of human FADD, and near the amino terminus of human DcR3, respectively. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC) resulting in activation of caspases. Death receptor signaling is also controlled by a family of decoy receptors (DcR1, DcR2, and DcR3) which lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand (3-5). The RIP (receptor-interacting protein) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that can trigger pro-survival and inflammatory responses through the activation of NF-κB as well as pro-apoptotic pathways (6). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and for the recruitment to TNFR1 through interaction with TRADD (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (7,8). Caspase-8 dependent cleavage of the death domain on RIP can trigger the apoptotic activity of RIP (9). RIP-deficient cells show a failure in TNF-mediated NF-κB activation, making the cells more sensitive to apoptosis (10,11).
- Nagata, S. (1997) Cell 88, 355-65.
- Thorburn, A. (2004) Cell Signal 16, 139-44.
- Sheridan, J.P. et al. (1997) Science 277, 818-21.
- Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6.
- Pitti, R.M. et al. (1998) Nature 396, 699-703.
- Meylan, E. and Tschopp, J. (2005) Trends Biochem Sci 30, 151-9.
- Hsu, H. et al. (1996) Immunity 4, 387-96.
- Stanger, B.Z. et al. (1995) Cell 81, 513-23.
- Lin, Y. et al. (1999) Genes Dev 13, 2514-26.
- Ting, A.T. et al. (1996) EMBO J 15, 6189-96.
- Kelliher, M.A. et al. (1998) Immunity 8, 297-303.
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- 9665 Caspase-3 (8G10) Rabbit mAb
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- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
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- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7722 Blue Loading Buffer Pack
- 7723 Red Loading Buffer Pack
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.