Product Pathways - MAPK Signaling
RSK1 (D6D5) Rabbit mAb #8408
PhosphoSitePlus® protein, site, and accession data: p90RSK
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk B Pg (C) (Dg) (Hr) | Endogenous | 90 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
B=Bovine
Dg=Dog
Pg=Pig
Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
RSK1 (D6D5) Rabbit mAb recognizes endogenous levels of total RSK1 protein. This antibody does not cross-react with other RSK isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu718 of human RSK1 protein.
Western Blotting
Western blot analysis of extracts from various cell lines using RSK1 (D6D5) Rabbit mAb.
Isoform Specificity
Western blot analysis of recombinant GST-tagged RSK1, RSK2, and RSK3 (2 μg each) using RSK1 (D6D5) Antibody #8408 (left panel), RSK2 (D21B2) Rabbit mAb #5528 (center panel) or GST Rabbit mAb #2625 (right panel).
Background
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).
- Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
- Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
Application References
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Companion Products
- 9333 RSK1 Antibody
- 9340 RSK2 Antibody
- 9343 RSK3 Antibody
- 9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb
- 9347 RSK1/RSK2/RSK3 Antibody
- 9335 Phospho-p90RSK (Ser380) (9D9) Rabbit mAb
- 9341 Phospho-p90RSK (Ser380) Antibody
- 9346 Phospho-p90RSK (Thr573) Antibody
- 3556 Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb
- 9348 Phospho-RSK3 (Thr356/Ser360) Antibody
- 9326 p90RSK Antibody Sampler Kit
- 7966 PathScan® Total RSK1 Sandwich ELISA Kit
- 7965 PathScan® Phospho-RSK1 (Ser380) Sandwich ELISA Kit
- 6309 SignalSilence® RSK1 siRNA I
- 6313 SignalSilence® RSK1 siRNA II
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
For Research Use Only. Not For Use In Diagnostic Procedures.
