Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb #8438

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC F H M R (Mk) Endogenous 18 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mab recognizes endogenous levels of caspase-7 protein only when cleaved at Asp198.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp198 of human caspase-7 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, C2C12, and H-4-II-E cells, untreated (-) or treated (+) with Staurosporine #9953 (1 μM, 3 hr), using Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb (upper) and Caspase-7 Antibody #9492 (lower).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 3 hr; right), using Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).

  1. Fernandes-Alnemri, T. et al. (1995) Cancer Res. 55, 6045-6052.
  2. Duan, H. et al. (1996) J. Biol. Chem. 271, 1621-1625.
  3. Lippke, J. A. et al. (1996) J. Biol. Chem. 271, 1825-1828.
  4. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  5. Thornberry, N. A. et al. (1997) J. Biol. Chem. 272, 17907-17911.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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