Cell Signaling Technology

Product Pathways - Neuroscience

SLC1A4 Antibody #8442

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 60-80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SLC1A4 Antibody recognizes endogenous levels of total SLC1A4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SLC1A4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain and human cerebellum using SLC1A4 Antibody.

Background

SLC1A4, also known as ASCT1, is a neutral amino acid transporter. Its other name, ASCT1, was given because it mediates obligatory exchange of alanine, serine, cysteine, and threonine (1). SLC1A4 mediates the efflux of glutamate from the neuron into the synaptic junction via calcium-independent release, as well as mediating the efflux of L-serine from glial cells and its uptake by neurons (2). SLC1A4-mediated transport is shown to involve a symmetrical potassium-independent electroneutral exchange of neutral amino acids and sodium, such that the current activated during transport is carried only by chloride ions (3).

  1. Takasaki, C. et al. (2007) Biomed Res 28, 61-9.
  2. Deng, X. et al. (2008) BMC Psychiatry 8, 58.
  3. Zerangue, N. and Kavanaugh, M.P. (1996) J Biol Chem 271, 27991-4.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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