Product Pathways - Translational Control
hnRNP A1 (D21H11) Rabbit mAb #8443
|8443S||100 µl (10 western blots)||---||In Stock||---|
|8443||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||34, 40||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
hnRNP A1 (D21H11) Rabbit mAb recognizes endogenous levels of total hnRNP A1 protein. This antibody does not cross react with other hnRNP family member proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly209 of human hnRNP A1 protein.
Western blot analysis of extracts from various cell lines using hnRNP A1 (D21H11) Rabbit mAb.
Flow cytometric analysis of HeLa cells using hnRNP A1 (D21H11) Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
Confocal immunofluorescent analysis of HeLa cells using hnRNP A1 (D21H1) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). hnRNP A1 regulates the alternative splicing of c-Src and c-H-Ras (2,3) and modifies initiation of translation of the fibroblast growth factor 2 mRNA (4). hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells (5). Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus (6,7). hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production (8,9). HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm (10).
- Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82.
- Rooke, N. et al. (2003) Mol Cell Biol 23, 1874-84.
- Guil, S. et al. (2003) Mol Cell Biol 23, 2927-41.
- Bonnal, S. et al. (2005) J Biol Chem 280, 4144-53.
- Patry, C. et al. (2003) Cancer Res 63, 7679-88.
- Piñol-Roma, S. and Dreyfuss, G. (1992) Nature 355, 730-2.
- Siomi, M.C. et al. (1997) J Cell Biol 138, 1181-92.
- Black, A.C. et al. (1996) Virus Genes 12, 275-85.
- Hadian, K. et al. (2009) J Biol Chem 284, 33384-91.
- Monette, A. et al. (2009) J Biol Chem 284, 31350-62.
- Thedieck, K. et al. (2013) Cell 154, 859-874. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.