Product Pathways - Growth Factors/Cytokines
Human C-C Motif Chemokine 3 (hCCL3/MIP-1-α) #8445
PhosphoSitePlus® protein, site, and accession data: CCL3
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Source
Recombinant human CCL3 (hCCL3) Ala27-Ala92 (Accession #NP_002974) was produced in E. coli at Cell Signaling Technology.
Molecular Characterization
Recombinant hCCL3 does not have a Met on the amino terminus and has a calculated MW of 7445. DTT-reduced and non-reduced protein migrate as 8 kDa polypeptides. The expected amino-terminal ADTPT of recombinant hCCL3 was verified by amino acid sequencing.
Purity
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCCL3. All lots are greater than 98% pure.
Bioactivity
The activity of hCCL3 was determined using a THP-1 cell migration assay. The ED50 of each lot is between 0.5-1.2 ng/ml.
Coomassie Gel
The purity of recombinant hCCL3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hCCL3 and staining overnight with Coomassie Blue.
Bioactivity
hCCL3-induced migration of THP-1 cells was asssessd. THP-1 cells were incubated in a 96-well transwell plate with increasing concentrations of hCCL3 in the bottom chamber. After 2 hr, the number of THP-1 cells that migrated to the bottom chamber of the transwell was quantified by measuring DNA content using a fluorescent dye.
Endotoxin
Less than 0.01 ng endotoxin/1 μg hCCL3.
Formulation
With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hCCL3. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.
Background
CCL3, also know as MIP-1-α, or LD78α, is an 8 kDa member of the C-C family of chemokines, which play key roles in immune surveillance, inflammation, and infection (1). CCL3 shares 68% amino acid identity with CCL4 (MIP-1-β) and 94% amino acid identity with LD78β (1). CCL3 is produced by activated monocytes, activated macrophages, NK cells, and T cells among others (1). There are two receptors for CCL3: CCR1 and CCR5 (1). CCL3 inhibits R5 HIV-1 infection by competing with the virus for its co-receptor, CCR5 (1). CCL3 polymorphisms are associated with risk for the development of rheumatoid arthritis and ulcerative colitis (2,3).
- Menten, P. et al. (2002) Cytokine Growth Factor Rev 13, 455-81.
- Zhang, R. et al. (2010) Int J Immunogenet 37, 273-8.
- Li, K. et al. (2009) Int J Colorectal Dis 24, 13-7.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
