Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

R-Ras Antibody #8446

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 24 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

R-Ras Antibody recognizes endogenous levels of total R-Ras protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human R-Ras protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using R-Ras Antibody.

Background

R-Ras, a member of the small GTPase family, is homologous to H-, K- and N-Ras, but does not activate MAP kinase pathways and is only weakly oncogenic (1). Instead, R-Ras is engaged in integrin activation (2). The effector loop and the carboxy-terminal proline-rich and prenylation sites of R-Ras are critical for integrin activation (3,4). Phosphorylation by EphB2 receptor tyrosine kinase and Src at Tyr66 of R-Ras suppresses integrin activity (5,6).

  1. Self, A. J. et al. (2001) J. Cell Sci. 114, 1357-1366.
  2. Zhang, Z. et al. (1996) Cell 85, 61-69.
  3. Oertli, B. et al. (2000) Oncogene 19, 4961-4969.
  4. Wang, B. et al. (2000) J Biol Chem 275, 5222-7.
  5. Zou, J.X. et al. (1999) Proc Natl Acad Sci U S A 96, 13813-8.
  6. Zou, J.X. et al. (2002) J Biol Chem 277, 1824-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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