Product Pathways - Metabolism
StAR (D10H12) XP® Rabbit mAb #8449
PhosphoSitePlus® protein, site, and accession data: StAR
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M R | Endogenous | 28 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
StAR (D10H12) XP® Rabbit mAb recognizes endogenous levels of total StAR protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg272 of human StAR protein.
Western Blotting
Western blot analysis of extracts from MLTC-1 cells and human, mouse, and rat adrenal tissue using StAR (D10H12) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of MLTC-1 cells (left) and C2C12 cells (right) using StAR (D10H12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Steroidogenic acute regulatory protein (StAR) plays a significant role in cholesterol transport from the cytoplasmic outer membrane to the inner mitochondrial membrane (1). The 37 kDa precursor is cleaved to generate an active 28 kDa protein capable of facilitating cholesterol metabolism into pregnenolone (2,3). StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue (3). Abnormalities in StAR gene expression are impacted in autosomal Lipoid Congenial Adrenal Hyperplasia (LCAH) resulting in defects in pregnenolone and cortisol synthesis (4). The mechanism of cholesterol binding to StAR has yet to be elucidated (4).
- Caron, K.M. et al. (1997) Proc Natl Acad Sci U S A 94, 11540-5.
- Bose, H.S. et al. (1999) Proc Natl Acad Sci U S A 96, 7250-5.
- Manna, P.R. et al. (2002) Biol Reprod 67, 1393-404.
- Roostaee, A. et al. (2008) Biochem J 412, 553-62.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
