Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

INPP4b (D19B9) Rabbit mAb #8450

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Endogenous 110 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

INPP4b (D19B9) Rabbit mAb recognizes endogenous levels of total INPP4b protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human INPP4b protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and RPMI 8226 cells using INPP4b (D19B9) Rabbit mAb.

Background

Phosphotidylinositol lipids and phosphoinositides are important second messengers, their generation controlling many cellular events. Intracellular levels of these molecules are regulated by phosphoinositide kinases and phosphatases. One of the best characterized lipid kinases is phosphoinositide 3-kinase (PI3K), which is responsible for phosphorylation on the D-3 position of the inositide head group (1). This action of PI3K catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN, the well characterized partnering phosphatase, reverses this process by removing the phosphate from PI(3,4,5)P3 at the D-3 position to generate PI(4,5)P2 (1,2). Dephosphorylation on the D-5 position to generate PI(3,4)P2 occurs through the action of SHIP1 or SHIP2 (3), and dephosphorylation on the D-4 position to generate PI(3)P can occur through the action of inositol polyphosphate 4-phosphatase isoenzymes type I (INPP4a) and type II (INPP4b) (4,5). While INPP4a has been implicated in neuronal survival and megakaryocyte lineage determination (6,7), less is understood about INPP4b. It has been shown that two splice variants of INPP4b occur in mice, each showing distinct tissue distribution and subcellular localization (5,8).

  1. Cantley, L.C. (2002) Science 296, 1655-7.
  2. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
  3. Ware, M.D. et al. (1996) Blood 88, 2833-40.
  4. Norris, F.A. et al. (1995) J Biol Chem 270, 16128-33.
  5. Norris, F.A. et al. (1997) J Biol Chem 272, 23859-64.
  6. Nystuen, A. et al. (2001) Neuron 32, 203-12.
  7. Vyas, P. et al. (2000) Proc Natl Acad Sci USA 97, 13696-701.
  8. Ferron, M. and Vacher, J. (2006) Gene 376, 152-61.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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