Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

Sall4 (D16H12) Rabbit mAb #8459

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H Endogenous 80, 142 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Sall4 (D16H12) Rabbit mAb recognizes endogenous levels of total Sall4A and Sall4B proteins. Western blot analysis detects additional bands that are likely Sall4 isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala311 of human Sall4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT cells using Sall4 (D16H12) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa cells (right) using Sall4 (D16H12) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

Members of the SALL gene family encode putative zinc finger transcription factors highly expressed during development (1). Sall4 is expressed very early in development with other pluripotency regulators, such as Oct-4 and Nanog (2). Recent studies suggest Sall4 works as a master regulator that controls its own expression and the expression of Oct-4 in a transcriptional regulation feedback loop governing stem cell pluripotency and stem cell fate (2,3). Immunohistochemical studies indicate that Sall4 is a sensitive and specific diagnostic marker for primary germ cell tumors and yolk sac tumors (4,5). Research studies have shown that Sall4 is constitutively expressed in acute myeloid leukemia (AML) and is a probable effector of the Wnt/β-catenin signaling pathway in this disease (6). In addition, mutations in Sall4 have been implicated in human malformation syndromes including Duane-radial ray syndrome (Okihiro syndrome) and Acro-renal-ocular syndrome (7).

  1. Sweetman, D. and Münsterberg, A. (2006) Dev Biol 293, 285-93.
  2. Yang, J. et al. (2008) Proc Natl Acad Sci USA 105, 19756-61.
  3. Yang, J. et al. (2010) PLoS One 5, e10766.
  4. Mei, K. et al. (2009) Mod Pathol 22, 1628-36.
  5. Miertus, J. et al. (2006) Hum Genet 119, 154-61.
  6. Ma, Y. et al. (2006) Blood 108, 2726-35.
  7. Al-Baradie, R. et al. (2002) Am J Hum Genet 71, 1195-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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