Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human His64­1BB Ligand/TNFSF9 (hHis64-1BBL) #8460

Request pricing for multi-milligram amounts.

Source

Recombinant human His64-1BBL (hHis64-1BBL) Arg71-Glu254 (Accession #NP_003802) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant N-terminally His6-tagged h4-1BBL has a calculated MW of 22,020. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides. The expected amino terminus of recombinant hHis64-1BBL was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hHis64-1BBL. All lots are greater than 98% pure.

Bioactivity

The activity of hHis64-1BBL was assessed by its ability to bind to 4-1BB in a functional ELISA. The concentration at which half-maximal binding was observed for each lot was 6-18 ng/ml.

Coomassie Gel

Coomassie Gel

The purity of recombinant hHis64-1BBL was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hHis64-1BBL and staining overnight with Coomassie Blue.

Bioactivity

Bioactivity

The activity of hHis64-1BBL was assessed by its ability to bind to 4-1BB in a functional ELISA.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hHis64-1BBL.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hHis64-1BBL. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

4-1BBL (also known as TNFSF9) is a member of the TNF-R superfamily, which includes OX40L, CD27L, CD40L, and GITRL (1). 4-1BBL is expressed primarily on antigen presenting cells, such as macrophages, dendritic cells, and B cells (2). 4-1BBL binds to 4-1BB on the surface of activated T cells (1). 4-1BBL/4-1BB binding activates the NF-κB pathway via TRAF1, 2, and 3 (1). 4-1BBL functions as a co-stimulatory molecule, inducing T cell proliferation, cytolytic activity, and cytokine production (2). Matrix metalloproteases can generate soluble 4-1BBL by cleaving it from the cell surface (3). The in vitro activity of soluble 4-1BBL is enhanced by cross-linking (4,5).

  1. Vinay, D.S. and Kwon, B.S. (2009) Cell Biol Int 33, 453-65.
  2. Wen, T. et al. (2002) J Immunol 168, 4897-906.
  3. Salih, H.R. et al. (2001) J Immunol 167, 4059-66.
  4. Rabu, C. et al. (2005) J Biol Chem 280, 41472-81.
  5. Wyzgol, A. et al. (2009) J Immunol 183, 1851-61.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products