Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Twinfilin-1 Antibody #8461

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Twinfilin-1 Antibody recognizes endogenous levels of total twinfilin-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human twinfilin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Twinfilin-1 Antibody.

Background

Twinfilin is an actin monomer-binding protein found in all eukaryotes (1). Mammals have three isoforms. Twinfilin-1 and twinfilin-2a are expressed in most non-muscle cell types, whereas twinfilin-2b is the main isoform in adult heart and skeletal muscle (2). Twinfilins are composed of two ADF-homology domains connected by a 30 kDa linker region. All twinfilins have been shown to form a 1:1 complex with G-actin, but not F-actin (reviewed in 3). Twinfilin-1 was originally known as A6 protein tyrosine kinase and thought to be part of a novel class of protein kinases. However, the protein was renamed after further studies showed no evidence of tyrosine kinase activity (4). Twinfilin-1 helps to prevent the actin filament assembly by forming a complex with actin monomers and, in mammals, has been shown to cap the filament barbed ends. It has been suggested that this regulates cell motility (5). Suppression of twinfilin-1 has also been shown to slow lymphoma cell migration to lymph nodes (6).

  1. Goode, B.L. et al. (1998) J Cell Biol 142, 723-33.
  2. Nevalainen, E.M. et al. (2009) Biochem J 417, 593-600.
  3. Palmgren, S. et al. (2002) J Cell Sci 115, 881-6.
  4. Vartiainen, M. et al. (2000) Mol Cell Biol 20, 1772-83.
  5. Helfer, E. et al. (2006) EMBO J 25, 1184-95.
  6. Meacham, C.E. et al. (2009) Nat Genet 41, 1133-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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