Cell Signaling Technology

Product Pathways - Protein Stability

CYLD (D1A10) Rabbit mAb #8462

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk (Hm) (B) (Dg) (GP) (Hr) Endogenous 108 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine  Dg=Dog  GP=Guinea Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CYLD (D1A10) Rabbit mAb recognizes endogenous levels of total CYLD protein. This antibody detects an unidentified protein of approximately 28 kDa in some cell types.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys37 of human CYLD protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CYLD (D1A10) Rabbit mAb.

Background

CYLD is a cytoplasmic deubiquitinating enzyme encoded by a tumor suppressor gene altered in individuals diagnosed with cylindromatosis, a genetic condition characterized by benign tumors of skin appendages (1,2). Functional CYLD deubiquitinase regulates inflammation and cell proliferation by down regulating NF-κB signaling through removal of ubiquitin chains from several NF-κB pathway proteins (3,4). CYLD is a negative regulator of proximal events in Wnt/β-catenin signaling and is a critical regulator of natural killer T cell development (5,6). The transcription factor Snail can inhibit CYLD expression, resulting in melanoma cell proliferation (7).

  1. Biggs, P.J. et al. (1995) Nat Genet 11, 441-3.
  2. Sun, S.C. (2010) Cell Death Differ 17, 25-34.
  3. Regamey, A. et al. (2003) J Exp Med 198, 1959-64.
  4. Glittenberg, M. and Ligoxygakis, P. (2007) Fly (Austin) 1, 330-2.
  5. Tauriello, D.V. et al. (2010) Mol Cell 37, 607-19.
  6. Lee, A.J. et al. (2010) EMBO J 29, 1600-12.
  7. Massoumi, R. et al. (2009) J Exp Med 206, 221-32.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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