Product Pathways - Adhesion
Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb #8467
|8467S||100 µl (10 western blots)||---||In Stock||---|
|8467||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, Dog||Endogenous||78||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142 and Ser143.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser142 and Ser143 of human zyxin protein.
Western blot analysis of extracts from A-431 and U-2 OS cells, untreated (-) or treated (+) with λ and calf intestinal phosphatase (λ/CIP), using Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb (upper) or Zyxin Antibody #3553 (lower).
The zyxin family of proteins includes LIMD1, ajuba, trip6 and zyxin, each of which contains three LIM domains at the carboxy-terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1).
Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2), and in regulation of adhesion and migration, possibly through recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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