Product Pathways - Adhesion
Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb #8467
PhosphoSitePlus® protein, site, and accession data: zyxin
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk Dg | Endogenous | 78 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142 and Ser143.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser142 and Ser143 of human zyxin protein.
Western Blotting
Western blot analysis of extracts from A-431 and U-2 OS cells, untreated (-) or treated (+) with λ and calf intestinal phosphatase (λ/CIP), using Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb (upper) or Zyxin Antibody #3553 (lower).
IP
Immunoprecipitation of extracts from A-431 cells using Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb. Western blot was performed using the same antibody. Lane 1 is 10% input.
Background
The zyxin family of proteins includes LIMD1, ajuba, trip6 and zyxin, each of which contains three LIM domains at the carboxy-terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1).Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2), and in regulation of adhesion and migration, possibly through recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4).
- Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
- Hirata, H. et al. (2008) J Cell Sci 121, 2795-804.
- Hoffman, L.M. et al. (2006) J Cell Biol 172, 771-82.
- Yu, Y.P. and Luo, J.H. (2006) Cancer Res 66, 7414-9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3553 Zyxin Antibody
- 4897 Ajuba Antibody
- 3389 LPP (8B3A11) Mouse mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
