Product Pathways - Neuroscience
Munc18-1 Antibody #8474
|W||H M R (Mk)||Endogenous||70||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Munc18-1 Antibody recognizes endogenous levels of total Munc18-1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near Asp453 of human Munc18-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Munc18-1 belongs to the Munc18 protein family which also includes Munc18-2 and Munc18-3. These proteins are highly conserved at the amino acid sequence level and play a critical role in membrane fusion. Munc18 proteins control SNARE complex formation (1,2) and interact with syntaxin (3). In Munc18-1 knockout mice, neurotransmitter release is completely abolished (4). In addition, Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane (5). Munc18-1 also acts as a regulator of vesicle docking during exocytosis (6).
- Rizo, J. and Südhof, T.C. (2002) Nat Rev Neurosci 3, 641-53.
- Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474-7.
- Han, G.A. et al. (2010) J Neurochem 115, 1-10.
- Verhage, M. et al. (2000) Science 287, 864-9.
- Medine, C.N. et al. (2007) J Cell Sci 120, 4407-15.
- Voets, T. et al. (2001) Neuron 31, 581-91.
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For Research Use Only. Not For Use In Diagnostic Procedures.