Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

Mic-1 (D2A3) Rabbit mAb #8479

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H (Mk) Endogenous 35, 13 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Mic-1 (D2A3) Rabbit mAb recognizes endogenous levels of total Mic-1 protein, including the processed mature form.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Mic-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1080 and Caki cells using Mic-1 (D2A3) Rabbit mAb.

Background

Macrophage inhibitory cytokine-1 (Mic-1), also termed GDF15 (1), PTGF-β (2), PLAB (3), PDF (4), and NAG-1 (5), is a divergent member of the transforming growth factor-β (TGF-β) superfamily (6). Like other family members, Mic-1 is synthesized as an inactive precursor that undergoes proteolytic processing involving removal of an N-terminal hydrophobic signal sequence followed by cleavage at a conserved RXXR site generating an active C-terminal domain that is secreted as a dimeric protein. Mic-1 is highly expressed in the placenta and is also dramatically increased by cellular stress, acute injury, inflammation, and cancer. In the brain, Mic-1 is found in the choroid plexus and is secreted into the cerebrospinal fluid (7). It is also a transcriptional target of the p53 tumor suppressor protein and may serve as a biomarker for p53 activity (8,9). During tumor progression, Mic-1 has various effects on apoptosis, differentiation, angiogenisis, and metastasis, and may also contribute to weight loss during cancer (10,11).

  1. Strelau, J. et al. (2000) J Neurosci 20, 8597-603.
  2. Yokoyama-Kobayashi, M. et al. (1997) J Biochem 122, 622-6.
  3. Hromas, R. et al. (1997) Biochim Biophys Acta 1354, 40-4.
  4. Paralkar, V.M. et al. (1998) J Biol Chem 273, 13760-7.
  5. Baek, S.J. et al. (2001) J Biol Chem 276, 33384-92.
  6. Bootcov, M.R. et al. (1997) Proc Natl Acad Sci USA 94, 11514-9.
  7. Strelau, J. et al. (2000) J Neural Transm Suppl , 273-6.
  8. Kannan, K. et al. (2000) FEBS Lett 470, 77-82.
  9. Yang, H. et al. (2003) Mol Cancer Ther 2, 1023-9.
  10. Johnen, H. et al. (2007) Nat Med 13, 1333-40.
  11. Bauskin, A.R. et al. (2006) Cancer Res 66, 4983-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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