Cell Signaling Technology

Product Pathways - Translational Control

IMP1 (D33A2) Rabbit mAb #8482

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 64 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IMP1 (D33A2) Rabbit mAb recognizes endogenous levels of total IMP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IMP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IMP1 (D33A2) Rabbit mAb.

Background

Insulin-like growth factor-II mRNA-binding proteins (IMPs) belong to a family of zipcode-binding proteins (1,2). Three members of this family, IMP1, IMP2, and IMP3, have been identified (1,2). They contain two RNA recognition motifs, four K homology domains, and were found to function in mRNA localization, turnover, and translation control (1,2). Research studies have implicated these proteins in a variety of physiological and pathological processes, such as growth and development (3), testicular neoplasia (4), and melanocytic neoplasia (5).

  1. Nielsen, F.C. et al. (2002) J Cell Sci 115, 2087-97.
  2. Nielsen, J. et al. (2003) Biochem J 376, 383-91.
  3. Hansen, T.V. et al. (2004) Mol Cell Biol 24, 4448-64.
  4. Hammer, N.A. et al. (2005) Reproduction 130, 203-12.
  5. Pryor, J.G. et al. (2008) Mod Pathol 21, 431-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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