Product Pathways - Neuroscience
Nestin (Rat-401) Mouse mAb (Alexa Fluor® 647 Conjugate) #8489
Reactivity Key: R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Nestin (Rat-401) Mouse mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of nestin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.
Confocal immunofluorescent analysis of P1 rat cerebellum (upper) and adult rat cerebellum (lower) using Nestin (Rat-401) Mouse mAb (Alexa Fluor® 647 Conjugate) (red) and Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #8024 (green). Blue pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for immunofluorescent analysis in rat brain. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nestin (Rat-401) Mouse mAb #4760.
Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.
- Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
- Giliarov, A.V. (2007) Morfologiia 131, 85-90.
- Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.
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For Research Use Only. Not For Use In Diagnostic Procedures.