Cell Signaling Technology

Product Pathways - Neuroscience

PEN2 (D2G6) Rabbit mAb #8502

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 13 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PEN2 (D2G6) Rabbit mAb recognizes endogenous levels of total PEN2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PEN2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PEN2 (D2G6) Rabbit mAb.

Background

Presenilin Enhancer 2 (PEN2) is a small integral membrane glycoprotein that contains two recognized transmembrane domains. Both the N- and C-terminal domains are oriented into the lumen of the endoplasmic reticulum (1). PEN2, along with Presenilin 1, Presenilin 2, Nicastrin, and APH-1 form the protein complex γ-secretase (2). The proteinase BACE catalyses the initial step in APP processing by cleaving and releasing soluble APPβ (3). The remaining membrane bound APP is then cleaved by the γ-secretase complex, causing the release of amyloid β-peptide, the main constituent of amyloid plaques. These plaques are a hallmark of Alzheimer’s disease pathology (2). In addition to APP, the γ-secretase complex cleaves several other proteins and necessary presenilin-dependent signaling cascades, including the Notch pathway (4). It was found that PEN2 is an important part of the γ-secretase complex, and knocking it down results in reduced amounts of the complex, resulting in a loss of γ-secretase activity (5).

  1. Sala Frigerio, C. et al. (2005) J Neurol 252, 1033-6.
  2. Hansson, C.A. et al. (2004) J Biol Chem 279, 51654-60.
  3. Hunt, C.E. and Turner, A.J. (2009) FEBS J 276, 1845-59.
  4. St George-Hyslop, P. and Schmitt-Ulms, G. (2010) Nature 467, 36-7.
  5. Steiner, H. et al. (2002) J Biol Chem 277, 39062-5.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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