Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Myosin Light Chain 2 (D18E2) Rabbit mAb #8505

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 18 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Myosin Light Chain 2 (D18E2) Rabbit mAb recognizes endogenous levels of total myosin light chain 2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human myosin light chain 2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Myosin Light Chain 2 (D18E2) Rabbit mAb.

Background

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).

  1. Ikebe, M. and Hartshorne, D.J. (1985) J. Biol. Chem. 260, 10027-10031.
  2. Tan, J. L. et al. (1992) Annu. Rev. Biochem. 61, 721-759.
  3. Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806.
  4. Ikebe, M. et al. (2000) J. Biol. Chem. 262, 9569-9573.
  5. Satterwhite, L. L. et al. (1992) J. Cell Biol. 118, 595-605.
  6. Sanbe, A. et al. (1999) J. Biol. Chem. 274, 21085-21094.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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