Product Pathways - Cytoskeletal Signaling
Sec31A Antibody #8506
|8506S||100 µl (10 western blots)||---||In Stock||---|
|8506||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Rat, Monkey||Endogenous||95-140||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Sec31A Antibody recognizes endogenous levels of multiple isoforms of total Sec31A protein, ranging in sizes of 95-140 kDa.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro810 of human Sec31A protein. Antibodies are purified by protein A and peptide affinity chromatography.
The coat protein complex II (COPII) is composed of five cytosolic proteins and includes the Sec23/24 complex, the Sec13/31 complex, and Sar1. The COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex to form a pre-budding complex that directly binds target molecules (1-3). This pre-budding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec31 subunit of COPII coat interacts with Sec13 at the ER exit and is required for both vesicle formation and ER-Golgi transport. Two isoforms of human Sec31 have been identified, Sec31A and Sec31B, which share a sequence homology of 47.3% (6-8). Sec31A is ubiquitously expressed in tissues and organs, whereas Sec31B is enriched in brain and testis (7,8). In classical Hodgkin lymphoma, a novel fusion of Jak2 with Sec31A renders Jak2 constitutively active and subject to Jak2 inhibitor effects (9).
Multiple isoforms of Sec31A protein have been reported (10).
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- Van Roosbroeck, K. et al. (2011) Blood 117, 4056-64.
- Townley, A.K. et al. (2008) J Cell Sci 121, 3025-34.
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