Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

KIF3A (D7G3) Rabbit mAb #8507

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 80 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

KIF3A (D7G3) Rabbit mAb recognizes endogenous levels of total KIF3A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human KIF3A protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using KIF3A (D7G3) Rabbit mAb.

Background

Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g. proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for a variety of cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with a variety of pathologies, ranging from tumorigenesis to defects in higher order brain function, such as learning and memory (4-6).

Kinesin superfamily protein 3A (KIF3A) is a central component of the kinesin-2 protein complex (7). KIF3A and its paralog KIF3B bind to form a heterodimeric motor protein with ATP-dependent, plus-end-directed microtubule sliding activity (8). The tail domain of this heterodimer binds to kinesin-associated protein 3 (KAP3), which facilitates binding of the KIF3A/3B motor protein to its cargo (7,8). Recent studies in a variety of model organisms have demonstrated a critical role for kinesin-family proteins, including KIF3A, in the formation and function of cilia (9). Notably, KIF3A was shown to mediate cilia-dependent protein-protein interactions that function to transduce canonical Hedgehog signaling (10).

  1. Hirokawa, N. et al. (2009) Nat Rev Mol Cell Biol 10, 682-96.
  2. Yu, Y. and Feng, Y.M. (2010) Cancer 116, 5150-60.
  3. Park, J.J. et al. (2008) Mol Endocrinol 22, 989-1005.
  4. Hirokawa, N. et al. (2010) Neuron 68, 610-38.
  5. Yoshimura, Y. et al. (2010) Mol Cell Biol 30, 2206-19.
  6. Hirokawa, N. and Noda, Y. (2008) Physiol Rev 88, 1089-118.
  7. Haraguchi, K. et al. (2006) J Biol Chem 281, 4094-9.
  8. Yamazaki, H. et al. (1995) J Cell Biol 130, 1387-99.
  9. Zhao, C. et al. (2012) Proc Natl Acad Sci U S A 109, 2388-93.
  10. Humke, E.W. et al. (2010) Genes Dev 24, 670-82.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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